John Wilkes (1725–1797) was an English radical and libertine in the 18th century.
John Wilkes may also refer to:
- John Wilkes (banker), founder of the First National Bank of Charlotte
- John Wilkes (printer) (1750–1810), English printer, bookseller and stationer
- John Wilkes (archaeologist) (born 1936), British archaeologist and academic
- John E. Wilkes (1895–1957), vice admiral, U.S. Navy
- John Vaughan Wilkes (1902–1986), English educationalist and Anglican priest
- John Wilkes (train), the name given to a passenger train of the Lehigh Valley Railroad