This is a list of characters in animation that either self-identify as bisexual or have been identified by outside parties to be bisexual. Listed characters are either recurring characters, cameos, guest stars, or one-off characters. This article also includes characters in Japanese animation, otherwise known as anime. There are also corresponding lists of lesbian and gay animated characters.
For fictional characters in other parts of the LGBTQ community, see the lists of gay, trans, lesbian, non-binary, pansexual, asexual, and intersex characters.
The names are organized alphabetically by surname (i.e. last name), or by a single name if the character does not have a surname. If more than two characters are in one entry, the last name of the first character is used.