Back كيمياء نسيجية مناعية Arabic Imunohistohemija BS Immunohistoquímica Catalan Imunohistochemie Czech Immunohistokemi Danish Immunhistochemie German Ιστοχημεία Greek Inmunohistoquímica Spanish Immunohistokeemia Estonian ایمونوهیستوشیمی Persian
Immunohistochemistry (IHC) is a form of immunostaining. It involves the process of selectively identifying antigens (proteins) in cells and tissue, by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Albert Hewett Coons, Ernest Berliner, Norman Jones and Hugh J Creech was the first to develop immunofluorescence in 1941. This led to the later development of immunohistochemistry.[1][2]
Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. In some cancer cells certain tumor antigens are expressed which make it possible to detect. Immunohistochemistry is also widely used in basic research, to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.[3]
- ^ Ortiz Hidalgo C (2022), Del Valle L (ed.), "Immunohistochemistry in Historical Perspective: Knowing the Past to Understand the Present", Immunohistochemistry and Immunocytochemistry, Methods in Molecular Biology, vol. 2422, New York, NY: Springer US, pp. 17–31, doi:10.1007/978-1-0716-1948-3_2, ISBN 978-1-0716-1947-6, PMID 34859396, S2CID 244861186, retrieved 2024-02-22
- ^ "Immunohistochemistry: Origins, Tips, and a Look to the Future". The Scientist Magazine®. Retrieved 2024-02-22.
- ^ Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M (2012). "Applications of immunohistochemistry". Journal of Pharmacy and Bioallied Sciences. 4 (6): S307-9. doi:10.4103/0975-7406.100281. ISSN 0975-7406. PMC 3467869. PMID 23066277.