Back تنقية البروتين Arabic Prečišćavanje proteina BS Purificació de proteïnes Catalan Proteinreinigung German Purificación de proteínas Spanish خالصسازی پروتئین Persian Purification des protéines French Purification de proteinas Interlingua 단백질 정제 Korean Eiwitzuivering Dutch
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the specification of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Ideally, to study a protein of interest, it must be separated from other components of the cell so that contaminants will not interfere in the examination of the protein of interest's structure and function.[1] Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.
- ^ Berg, Jeremy M.; Tymoczko, John L.; Stryer, Lubert (2012). Biochemistry (7th ed.). New York: W. H. Freeman and Company. pp. 69–70. ISBN 978-1-4292-2936-4.